Seroprevalence of dengue infection in clinically suspected cases of dengue at tertiary care hospital in Gujarat

Abstract

Objective: Dengue virus is the most common arbo virus found in India. Our study has conducted to study the epidemiological and demographic characteristic of dengue fever during the year 2017 in Ahmedabad region of Gujarat state India. Materials and Methods: A retrospective study was performed at the Department of Microbiology, Dr. M.K.Shah Medical College and research centre and smt. S.M.S. multispeciality hospital Ahmedabad, Gujarat from July 2017 to October 2017. A total of 611 blood samples were collected during the study period and serologically tested for dengue NS1 antigen and IgM antibody by capture ELISA testing. Patient with clinically suspected dengue and found positive for dengue NS1 antigen and/or dengue IgM Ab was considered as a case. The data was entered in software and analyzed. Results: We have tested 611 serum samples for dengue infection during the study period in our hospital. Out of which 104(17%) serum samples were positive for dengue infection. 77 serum samples were positive for Dengue NS1 antigen ELISA and 27 serum samples are positive for Dengue IgM ELISA. Majority of samples were tested positive in age group 14-25 year (64.5%) and 26-60 year (32.7%). It shows prevalence of dengue infection is more in male (82.7%) than female (17.3%). Conclusion: Rapid urbanization in developing countries increases prevalence of dengue. Virus activity is high during monsoon and post monsoon period which co-insides with increase vector breeding. Effective implementation of vector control measures through efforts toward vector breeding source reduction help in reduction of the dengue prevalence in community. Keywords: Dengue, NS1 antigen, IgM antibody, Seroprevalence.