Seroprevalence of Dengue by Detection of NS1, IgM in Tertiary Care Hospital, RIMS, Ranchi

Abstract

Introduction: Dengue virus being most significant among vector borne diseases is leading to increased morbidity and mortality in India. Global prevalence of dengue has been increasing over the last decade. So, it is important to screen all dengue fever like cases. Objective: The study was conducted to know seroprevalence of Dengue virus by detecting NS1, IgM in clinically suspected dengue fever cases. Material and Method: Test was done in 315 blood samples of clinically suspected dengue fever cases, in various departments of RIMS, Ranchi. The study was done from November 2016 to August 2017. NS1 antigen and IgM antibody detection both were done by ELISA (NS1 by Qualisa Microwell Enzyme Immunoassay and IgM by NIV Dengue IgM Captured ELISA KIT). Result and Discussion: Out of 315 blood samples, 84 samples (26.7%) were serologically positive for dengue infection. Among the positive cases 72.6% were males and 27.4% were females. Males are more affected than females, because males are more exposed during their outdoor visit for livelihood. Age group between 21-30 years were most affected. Most of the cases were reported in monsoon and post- monsoon season. Conclusion: This study highlights the need of continuous surveillance by both NS1 antigen and IgM antibody by ELISA method for early detection of impending outbreak to initiate timely prevention and control measures. Keywords: Dengue, NS1, IgM.