Abstract
Brucellosis has been an emerging, zoonotic disease that is associated with chronic debilitating infections in humans and reproductive failure in domestic animals. The aim of this study was to investigate the seroprevalence of brucellosis in samples collected from cattle in Swat valley based on the Rose Bengal precipitation test (RBPT), milk ring test (MRT), and indirect enzyme-linked immunosorbent assay (i-ELISA for both milk and serum). A total number 0f 310 samples were processed during the study which consists of 200 serum samples and 110 milk samples. The 200 serum samples were collected from male and female cattle (n=11and n=189).The RBPT and i-ELISA (serum) detected antibodies against B.abortus showed 1.587%, 2.12% in females through both tests respectively, and 0.0% in males on both techniques. The milk samples (n=110) detected B.abortus antibodies 0.9%, 1.82% through MRT and i-ELISA (milk) respectively. The comparative efficacy of MRT and i-ELISA (milk) was also analyzed statistically by z-test, the data revealed insignificant results (p= 0.561) also statistical analysis of RBPT and i-ELISA (serum) findings showed insignificant results (p= 0.703) therefore both results are higher than the significant level (0.05). Keywords: brucellosis; ELISA; Milk ring test; Rose Bengal plate test; Sero-prevalence http://dx.doi.org/10.19045/bspab.2021.100149
Highlights
Introduction infection in cattle named asBang's disease" is Brucellosis is an infectious zoonotic disease often shortened to just "bangs"
All the serum samples were tested through Rose bengal plate test (RBPT) and i-ELISA while milk samples were processed through Milk Ring Test (MRT) and i-ELISA
This study showed a greater incidence of Brucellosis in female cows (1.587%) than male cattle (0.0%)
Summary
Introduction infection in cattle named asBang's disease" is Brucellosis is an infectious zoonotic disease often shortened to just "bangs". Different assays can be used for screening mass-scale populations that includes SAT, Milk Ring Test (MRT), RBPT, and ELISA. The aim of this study was to look into the prevalence of B. abortus in cattle and to compare the efficacy of RBPT. MRT and I-ELISA for detection of antibodies against B. abortus were studied. All samples were subjected separately to serological tests and MRT for the detection of any positive case of brucellosis in target animals. 100 ul (1:50 in dilution buffer) serum sample, positive and negative control sera were added in the respective well pre-coated with B. abortus antigen followed by incubation at 37°C for an hour. A positive reaction was indicated by a stained cream layer over the white column of milk Results The study revealed that the prevalence 1.5% in cattle (n=200). The prevalence was 0.0% (n=11) but it was 1.587% in females (n=189) through RBPT
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