Seroprevalence of Bovine Leptospirosis using Indirect ELISA

Abstract

Background: Leptospirosis is a zoonotic bacterial disease causing abortion, stillbirths and loss of milk production in livestock resulting in huge economic losses. Leptospirosis in cattle has been under-reported and under-diagnosed in India due to non-specific symptoms, complex laboratory tests, fastidious nature of bacterium and lack of proper diagnostic facilities. Scanty data pertaining to epidemiological status of leptospirosis is available in land locked states of India including Madhya Pradesh, thus to fulfil the gap, the current work was conducted to investigate seroprevalence of leptospirosis in cattle. Methods: In the present study, cattle with a history of repeat breeding, abortion, haemogalactia and mastitis, were screened from various organized and unorganized farms of Jabalpur. A total of 300 blood samples were collected from suspected cattle and serum was separated. Enzyme linked Immune-sorbent assay was done as per the guidelines. Result: The seroprevalence of leptospirosis among suspected cases was 48.67 per cent, it was significantly higher in organized sector in cattle with the history of abortion followed by repeat breeding. High seropositivity was reported in older cattle.