Seroprevalence and risk factors for Neospora spp. infection in equine in Egypt

Abstract

Neospora infections in equine are associated with reproductive disorders and neurological diseases. Nevertheless, Egypt has no epidemiological information on this parasite in equine. This study determined the prevalence of Neospora spp. infection in 325 equines from three Egyptian governorates located at Northern Egypt using cELISA. The prevalence of antibodies against Neospora spp. was 19% (95% CI: 14.09–25.05) in horse, 34.1% (95%CI: 24.92–44.69) in donkey and 26.7% (95% CI: 15.97–41.04) in mule. In comparison to horse, donkey had a considerably higher chance of contracting Neospora spp. infection (OR = 1.80, 95% CI: 0.78–4.13; P = 0.016). The risk was also higher in freely grazing animals (OR = 3.49, 95% CI: 0.95–12.78; P = 0.059). Moreover, yearling animals (12–24 months) (OR = 5.03, 95% CI: 1.51–16.80; P = 0.009) and those with natural breeding (OR = 11.80, 95% CI: 3.24–42.99, P < 0.0001) and a history of early abortion (OR = 7.04, 95% CI: 3.01–16.47; P < 0.0001) also showed a greater risk of seropositivity. The risk of Neospora infection increased significantly in equines contacted with dogs (OR = 5.16, 95% CI: 1.76–15.10; P = 0.003). This the first serological study to determine the seroprevalence of Neospora spp. in equine in Egypt. Further studies are necessary to identify the species of Neospora and to understand the role of above-mentioned risk factors in spreading of infection.