Serological survey for avian influenza virus infection of backyard poultry and poultry workers in Baghdad and Basrah provinces, Iraq

Abstract

The aim of this study was to determine the prevalence of avian influenza virus (AIV) subtype H5N1 among backyard birds and poultry workers. The study included backyard birds (chickens and ducks) in rural areas of Baghdad and Basrah provinces, Iraq. Rural areas were divided into wet land and dry land. Total egg samples were 368, divided into 184 eggs (92 chicken eggs and 92 duck eggs) from each province. The total number of human sera was 180 (45 poultry worker and 45 non-poultry worker samples from each province). Competitive ELISA tests were used for detection of specific antibodies against influenza A virus and influenza subtype H5N1 in all samples. Seroprevalence against AIV type A in chickens was 62.5% (115/184) while ducks showed seroprevalence of 40.8% (75/184). The seropositivity among the backyard birds in wet land rural areas was higher than that in dry land rural areas. The samples positive for subtype H5N1 in chickens were 15.7% (18/115) while in ducks: 12% (9/75) with highest seropositivity among chickens in wet land rural areas (18.8%; 12/64). The seropositivity of avian influenza H5N1 was 6% (4/67) among AIV-positive poultry workers and 2.9% (1/34) among non-poultry workers.