SEROLOGICAL STUDIES ON CEREBROSPINAL FLUID

Abstract

By means of paper electrophoresis and reaction of the precipitation, the author attempted to detect whether the formation of cerebrospinal fluid protein was ascribed to blood or to the other part except blood. The results obtained were as follows: First, each protein fraction of cerebrospinal fluid was were extracted and their perfect pictures were taken. by means of paper electrophoresis Second, a comparison was made between normal cerebrospinal fluid and human serum by means of reaction of precipitation. As the result there found to be exist serologically same protein in quality with serum protein in normal cerebrospinal fluid, and this protein was contained in euglobulin fraction. Also, in nermal cerecrospinal fluid, serologically different protein from serum protein in quality was contained and this protein was proved in pseudoglobulin or in substance very resembled it. Then next to the foregoing study, an experiment was carried out as to the protein originated in the case of meningitis. The results were that the meningitic cerebrospinal fluid was removed into normal cerebrospinal fluid without accepting any changes for its serum components by normal cerebrospinal fluid and an extorordinally promoted permeability. Thus the meningitic cerebrospinal fluid was composed. The common protein ingredient between normal cerebrospinal fluid and meningitic cerebrospinal fluid was found that it was existing in albumin fraction, and also in meningitic cerebrospinal fluid, partially, peculiar kind protein ingredient which could not be found in human serum or normal cerebrospinal fluid were observed. It was considered that serum protein might be excreted after accepting the alteration in quality by choroid plexus. Furthermore, another experiment was carried out on the correlation between cerebrospinal fluid protein fraction fraction and serum protein fraction and in consequences it was found that each cerebrospinal fluid protein contained the protein in common to serum protein. Also, in appearance, the protein being contained in normal cerebrospinal fluid pas comparatively rich in euglobulin ingredient than those of albumin and pseudoglobulin. Meningitic cerebrospinal fluid equally contained albumin, euglobulin, and pseudoglobulin and it was found that its composition was very resemble that of serum protein.