Abstract

The isolation, purification, and serological properties of a 130-nm iridescent virus (IV), type 25, from Tipula sp. is reported. The polypeptide profile of this isolate was different from that of another small Dipterous iridescent virus-IV22 isolated from Simulium sp. However, these viruses were indistinguishable when compared by serum neutralization, gel immunodiffusion, complement fixation, tube immunoprecipitation, and immune electron microscopy. Iridescent virus type 2 isolated from Sericesthis pruinosa was shown to be distinct from IV22 and IV25 by all of these techniques.

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