Serological profiling of rabies antibodies by enzyme-linked immunosorbent assay and its comparative analysis with rapid fluorescent focus inhibition test in mouse model

Ashis Debnath,Dinesh C. Pathak,Narayan Ramamurthy,Vikramaditya Upmanyu,Sohini Dey,R. Saravanan,A. K. Tiwari,Madhan Mohan Chellappa,Gulam Mohd,A. B. Pandey

doi:10.14202/vetworld.2019.126-130

Ashis Debnath, Dinesh C. Pathak + Show 8 more

Open Access

https://doi.org/10.14202/vetworld.2019.126-130

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Journal: Veterinary world	Publication Date: Jan 1, 2019
Citations: 8	License type: CC BY 4.0

Affiliation: Indian Veterinary Research Institute

Abstract

Aim:In this study, we have used enzyme-linked immunosorbent assay (ELISA) as an alternative test to replace the cumbersome rapid fluorescent focus inhibition test (RFFIT) to ascertain the immune status of immunized mice against rabies virus.Materials and Methods:Rabies is a devastating disease worldwide caused by rabies virus. Proper usage of pre- or post-exposure rabies vaccine can prevent the disease transmission. In this study, mice were immunized with Vero cell-adapted inactivated rabies vaccine. RFFIT was used as a test to determine the serum neutralizing titers in infected/vaccinated mice. Seroprofiling of mice sera was done in vitro by ELISA.Results:Twenty-one days post-immunization, both ELISA and RFFIT assays indicated similar antibody levels in mice sera that were immunized with Vero cell-adapted inactivated rabies vaccine. Both the tests were correlated, and the linearity was verified by the regression line (R²=0.979).Conclusion:In this study, we profiled the serological status of Vero cell-adapted inactivated rabies vaccine through ELISA in mice model that correlated well with the OIE gold standard test RFFIT.

Highlights

In this study, we profiled the serological status of Vero cell-adapted inactivated rabies vaccine through enzyme-linked immunosorbent assay (ELISA) in mice model that correlated well with the OIE gold standard test rapid fluorescent focus inhibition test (RFFIT)
The level of the anti-rabies antibody was determined as mean absorbance at 490 nm and was compared to that of the cutoff value
Rabies is a fatal disease worldwide caused by rabies virus

Summary

Introduction

Rabies virus is a neurotropic, negative sense, single-stranded RNA virus that belongs to the family Rhabdoviridae, genus Lyssavirus [1]. The causative virus can spread from saliva of infected animals to others through neuromuscular route [2]. It has been reported that around 55,000 human deaths occur due to rabies infection out of the millions exposed each year worldwide [3]. The disease is endemic in nature and poses a risk to international travelers, in Asian countries [4,5,6,7,8,9]. The only way to prevent this disease is to get proper vaccination using pre- or post-exposure vaccines [10].

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