Abstract
Abstract : This study was conducted to investigate the status of brucellosis in dairy cattle from five selected dairyfarms in the Mohammadpur Beribadh area of Bangladesh. A cross-sectional study was carried out from October 2010to March 2011 in which a total of 334 serum samples from cattle in five herds were screened by the Rose-Bengalplate-agglutination test (RBPT) and the positives were confirmed using an indirect enzyme-linked immunosorbent assay(I-ELISA). A structured questionnaire was used to collect epidemiological information describing the animals. Overall,4.20% of the animals were RBPT positive, while subsequent confirmatory tests with I-ELISA revealed that the overallanimal-level prevalence derived from the samples was 1.20%. Additionally, the prevalence was relatively higher infemales than in males. A significant association was found between abortion, age of the animals, and the occurrenceof brucellosis (p < 0.05). Considering the overall low prevalence of brucellosis in the selected farms in the presentstudy, a brucellosis eradication program for dairy farms using a test-and-slaughter policy would be possible.Keywords : Bangladesh, brucellosis, indirect enzyme-linked immunosorbent assay, Rose-Bengal plate-agglutination test,seroprevalence
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