Abstract
Paratuberculosis a contagious and chronic disease in domestic and wild ruminants, is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Typical clinical signs include intractable diarrhea, progressive emaciation, proliferative enteropathy, and mesenteric lymphadenitis. Paratuberculosis is endemic to many parts of the world and responsible for considerable economic losses. In this study, different types of paratuberculosis and MAP in sheep and goats were investigated in Inner Mongolia, a northern province in China contiguous with two countries and eight other provinces. A total of 4434 serum samples were collected from six cities in the western, central, and eastern regions of Inner Mongolia and analyzed using the ELISA test. In addition, tissue samples were collected from seven animals that were suspected to be infected with MAP. Finally, these tissues samples were analyzed by histopathological examination followed by polymerase chain reaction (PCR), IS1311 PCR-restriction enzyme analysis (PCR-REA), and a sequence analysis of five genes. Among all 4434 ruminant serum samples collected from the six cities in the western, central, and eastern regions of Inner Mongolia, 7.60% (337/4434) measured positive for the MAP antibody. The proportions of positive MAP antibody results for serum samples collected in the western, central, and eastern regions were 5.10% (105/2058), 6.63% (85/1282), and 13.44% (147/1094), respectively. For the seven suspected infected animals selected from the herd with the highest rate of positivity, the gross pathology and histopathology of the necropsied animals were found to be consistent with the pathological features of paratuberculosis. The PCR analysis further confirmed the diagnosis of paratuberculosis. The rest of the results demonstrated that herds of sheep and goats in Inner Mongolia were infected with both MAP type II and type III. To the best of our knowledge, this is the first study of the two subtypes of MAP strains in sheep and goats in Inner Mongolia.
Highlights
Mycobacterium avium subsp. paratuberculosis (MAP) is a member of the Mycobacterium avium complex (MAC) and the causative agent of paratuberculosis (Johne’s disease)
Among all 4434 ruminant serum samples collected from the six cities in the western, central, and eastern regions of Inner Mongolia from 2018 to 2019, 7.60% (337/4434) tested positive for the MAP antibody
Significant proliferation of lymphocytes was observed in the entire lymph node and found to be the most prominent in the paracortical area
Summary
Mycobacterium avium subsp. paratuberculosis (MAP) is a member of the Mycobacterium avium complex (MAC) and the causative agent of paratuberculosis (Johne’s disease). Paratuberculosis (MAP) is a member of the Mycobacterium avium complex (MAC) and the causative agent of paratuberculosis (Johne’s disease). The infection primarily affects ruminants and the main signs of infection include diarrhea and wasting. This disease is endemic to many different counties and responsible for considerable economic losses [1,2,3]. Occasional asymptomatic infections have been found in wild animals [18] It remains controversial whether MAP is the causative agent of Crohn’s disease [3, 19], the presence of MAP in the food chain has been widely acknowledged and has received substantial attention in food industries. The rising awareness of MAP in the food chain has prompted measures for on-farm control of Johne’s disease
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
