Abstract
Summary. Isohemagglutinating activity of sera from group 0, Rh‐positive pregnant women were tested against infant's father erythrocytes using two agglutination techniques.Isohemagglutinin titers obtained by the dextran technique are considerably higher than those obtained by agglutination in saline. In samples of sera treated with mercapto‐ethanol this difference is even more marked.The presence of high isohemagglutinins titer in maternal pre‐partum sample of serum occurring together with a lower titer or absence of free isohemagglutinins in the corresponding umbilical cord serum has been found to be closely related to the severity of ABO hemolytic disease of the newborn.
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