Serological assays for the identification of Oesophagostomum dentatum infections in pigs

Abstract

Oesophagostomum dentatum antigen preparations of third (L 3) or fourth (L 4) stage larvae were characterised by Western blotting. Panels of sera obtained from pigs infected with O dentatum and Ascaris suum, respectively, reacted with the same bands of L 3 antigen. In contrast high specificity against a characteristic band, was observed when L 4 extract was employed as antigen. To establish an enzyme-linked immunosorbent assay ( ELISA), a panel of homologous and heterologous sera was tested against O dentatum L 4 extract. The best combined specificity and sensitivity was obtained when horseradish peroxidase ( HRP) goat anti swine IgG conjugate was used rather than HRP rabbit anti swine Ig conjugate. Testing series of sera from pigs infected with single doses of either 2000, 20,000 or 200,000 infective larvae by the ELISA, a significant dose dependency in the antibody response was observed between the low and high dosage groups. This assay may be useful in future studies of the immune-mechanisms against nodular worm infections in pigs.