Abstract
Prune dwarf virus (PDV) is one of the major positive RNA viruses which cause economical damages in stone fruit trees. The symptoms of PDV vary between different stone fruits namely sour and sweet cherry, almond, peach, apricot and plum including leaf narrowing, leaf chlorosis, vein clearing, mosaic, leaf whitening, leathery leaf, bushy branches and stunt trees. During the years 2011 and 2012, 251 leaf samples were collected for detection of PDV in stone fruit orchards of Charmahal-va-Bakhtiari province. DAS-ELISA test proved PDV presence serologically. Then, total RNA were extracted and tested by two-step RT-PCR which replicated partial and full coat protein sequence of PDV. One hundred and eighty one out of total samples (251 samples) showed PDV infection using serological and two-step RT-PCR assays, hence, incidence of PDV in Charmahal-va-Bakhtiari province was confirmed. This is the first report of PDV in stone fruit orchards of Charmahal-va-Bakhtiari province and in Iran.
Highlights
Mechanical transmissionNaN3, pH 9.8) was added to each well and incubated in a dark place at room temperature for Curcubita maxima, Chenopodium album, 30-60 min
ELISA Plate was pre-coated with coating antibody that was diluted in coating buffer (0.01 M Na2CO3, 0.03 M NaHCO3, 3 mM NaN3 per L, pH 9.6) at 1:1000, and incubated overnight at 4°C
Viral diseases are one of the reducing factors of stone fruits yield, which RNA viruses play an important role in this loss
Summary
NaN3, pH 9.8) was added to each well and incubated in a dark place at room temperature for Curcubita maxima, Chenopodium album, 30-60 min. Viral diseases are one of the reducing factors of stone fruits yield, which RNA viruses play an important role in this loss. Charmahalva-Bakhtiari province has great areas under stone fruits cultivation in which viral diseases decrease production and fruits quality. RNA viruses cause most of stone fruits economical damages. PDV whether solely or in mix infection causes loss of stone fruit prod-. Nicotiana tabacum var Turkish and N. rustica were inoculated by crude leaf homogenate of stone fruit leaves which were extracted in 0.1 M phosphate buffer, pH 7 containing 0.1% 2mercaptoethanol. The homogenate was rubbed on the leaves coated with carborundum powder. The inoculated plants were kept under observation for two weeks to record potential symptoms
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