Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt

Tamam S.M,Shokier K.A,El-Shereif N.M

doi:10.21608/jvmr.2018.43304

Abstract

lumpy skin disease virus (LSDV) was isolated, from naturally infected cattle that have a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine. The virus was isolated on chorio-allantoic membranes (CAM) of embryonated chicken eggs (ECE) and Madin Darby Bovine Kidney Cells (MDBK) and identified by agar gel precipitation test (AGPT) and immunofluorescent antibody technique (IFA). Characteristic pock lesions and intracyptoplasmic flourescene granules are identified respectively. Molecular characterization using polymerase chain reaction (PCR) using specific primer for G-Protein Coupled Chemokine Receptor Gene of LSDV isolates specific amplified product 554 bp. Sequence analysis revealed tow new isolates of LSDV.

Highlights

Lumpy skin disease (LSD) is an infectious, eruptive and occasionally fatal disease of cattle characterized by nodules formation in the skin, mucosal membranes, lymphadenopathy and occasionally death [1]
LSD is caused by the strain of capripoxvirus which is genetically and antigenically close related to the strain of sheep and goat pox virus and the prototype strain is known as the Neethling Pox virus [2]
Materials and Methods Samples: intracutaneous skin nodules were collected by biopsy from naturally infected cattle suspected to be infected with lumpy skin disease virus (LSDV) infection with a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine located at different areas in Beni-Suef Governorate

Summary

Introduction

LSD is caused by the strain of capripoxvirus which is genetically and antigenically close related to the strain of sheep and goat pox virus and the prototype strain is known as the Neethling Pox virus [2]. The disease is listed in the office international des Epizooties List “A” which identifies diseases with the potential rapid spread and severe economic losses [3]. In Egypt, LSDV was first isolated and identified from cattle during two outbreaks in Suez and Ismalia governorates in 1989 [4].The possible introduction of new strains of LSDV by the uninterrupted movement of animals across borders is a major constant threat. In early 2006, a severe LSD outbreak struck foreign (imported from Ethiopia) and local cattle in different Egyptian governorates, causing enormous economic losses. Control of LSDV in Egypt depends on prophylactic vaccination programs using live attenuated cell culture adapted sheep pox virus vaccine [5]

Objectives

Methods

Results