Abstract
The pattern of activity of some of the “DNA-synthesizing” enzymes was examined in rabbit kidney cells in the stationary phase of growth before and after infection with pseudorabies virus. It was found that the levels of activity of thymidine kinase, thymidine monophosphate kinase, and DNA polymerase, which are barely detectable in noninfected cells, start to increase between 1 and 2 hours after infection. No sequence in the time of appearance of these enzymes could be detected. The levels of activity of deoxyadenosine monophosphate kinase, deoxycytidine monophosphate kinase, and deoxyguanosine monophosphate kinase remain high in cells which reach the stationary phase of growth and are unchanged by infection. The antigenic relationships between thymidine kinase, DNA polymerase, and deoxyadenosine monophosphate kinase present in noninfected cell and in virus-infected cell extracts were determined by testing the effect of incubation with specific γ-globulins on the enzyme activity. These tests showed that the deoxyadenosine monophosphate DNA polymerases present in infected and noninfected cells are antigenically related. Thymidine kinase present in infected cells is, however, unrelated antigenically to the protein performing the same function in noninfected cells.
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