Abstract
We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.
Highlights
We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment
A) Validation of the specificity of the receptor-binding inhibition assay (RBI) assay for the detection of Middle East respiratory syndrome (MERS)-CoV–specific antibodies in humans
CoV, human coronavirus; HI, hemagglutination inhibition; MERS-CoV, Middle East respiratory syndrome coronavirus; PRNT90, 90% reduction in plaque reduction neutralization test; RBI, receptor-binding inhibition
Summary
GeurtsvanKessel, Elmoubasher A.B.A. Farag, Mohammed Al-Hajri, Wan Beom Park, Myoung-don Oh, Chantal B.E.M. Reusken, Marion P.G. Koopmans, Berend-Jan Bosch, Bart L. Author affiliations: Erasmus Medical Center, Rotterdam, the Netherlands (N.M.A. Okba, C.H. GeurtsvanKessel, C.B.E.M. Reusken, M.P.G. Koopmans, B.L. Haagmans); Utrecht University, Utrecht, the Netherlands We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment.
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