Serodiagnosis of Lumpy Skin Disease Using Sheep Pox Virus Compared to a Commercial ELISA Kit

Ayatollah I Ibrahim,Doaa I Rady,Eman R Abdo,Alaa A El-Kholy

doi:10.21608/javs.2021.103303.1112

Abstract

Serodiagnosis of Lumpy Skin Disease Using Sheep Pox Virus Compared to a Commercial ELISA Kit

Highlights

Lumpy skin disease (LSD) is a viral disease of cattle caused by the lumpy skin disease virus (LSDV) (OIE, 2018)
1.Sensitivity and specificity of Enzyme-Linked Immune Sorbent Assay (ELISA) and virus neutralization test (VNT) using negative and positive control samples: One hundred and fifty serum samples were divided into 75 serum samples collected from cattle showing different symptoms and stages of Lumpy Skin Disease (LSD) from mild to severe cases as shown in figure (1) and confirmed using Polymerase Chain Reaction (PCR) for detection of 192 bp, which is characteristic for LSDV as shown in figure (2) act as VNT using sheep pox virus (SPV) 67/75 (89.3%) 74/75 (98.6%)
Comparative mean reactivity of VNT using SPV and LSDV compared to ELISA Kit for LSD prepared hyperimmune sera and vaccinated bovine sera: By testing 75 serum samples weekly collected from 25 vaccinated calves, the results presented in table (2) show positive results for all serum samples by commercial ELISA KIT and VNT using LSDV along the period of study 2 weeks post-vaccination (WPV) till 4WPV with 100% positive while in case of VNT using SPV it was 80 % at 2WPV and 100 % at 3 and 4 WPV with overall 93%

Summary

Introduction

Lumpy skin disease (LSD) is a viral disease of cattle caused by the lumpy skin disease virus (LSDV) (OIE, 2018). LSDV is classified as a member of genus Capripoxvirus within a subfamily Chordopoxvirinae family Poxviridae together with goat pox (GP) and sheep pox (SP) viruses. Viruses of this genus share 97% of genomic nucleotide sequence identity, crossprotectivity; they are serologically indistinguishable as infection with one strain provides immunity against all other strains because of their homologous antigenicity. In Egypt, LSDV was isolated and identified for the first time during a devastating outbreak in Suez and Ismailia governorates in 1989 (House et al, 1990 and Davies, 1991). In early 2006, a severe LSD outbreak was introduced to Egypt by importing cattle from Ethiopia that affected the local bovine population in different governorates, causing severe economic

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Discussion

Conclusion