Abstract
The aims of the present work were to determine the prevalence and titer of serum antibodies against several rotavirus VP8* proteins from different P genotypes in children and adults in Valencia, Spain; and to determine the role of the secretor status (FUT2G428A polymorphism) in the antibody response. The VP8* protein from the P[4], P[6], P[8], P[9], P[11], P[14] and P[25] genotypes were produced in E. coli. These proteins were tested with 88 serum samples from children (n = 41, 3.5 years old in average) and from adults (n = 47, 58 years old in average) by ELISA. A subset of 55 samples were genotyped for the FUT2G428A polymorphism and the antibody titers compared. The same subset of samples was also analysed by ELISA using whole rotavirus Wa particles (G1P[8]) as antigen. Ninety-three per cent of the samples were positive for at least one of the VP8* antigens. Differences in the IgG seroprevalence were found between children and adults for the P[4], P[8] and P[11] genotypes. Similarly, significant differences were found between adults and children in their antibody titers against the P[4], P[8], and P[11] VP8* genotypes, having the children higher antibody titers than adults. Interestingly, positive samples against rare genotypes such as P[11] (only in children), P[14] and P[25] were found. While no statistical differences in the antibody titers between secretors and non-secretors were found for any of the tested P genotypes studied, a higher statistic significant prevalence for the P[25] genotype was found in secretors compared to non-secretors. Significant differences in the antibody titers between secretors and non-secretors were found when the whole viral particles from the Wa rotavirus strain (G1P[8]) were used as the antigen.
Highlights
Rotaviruses are the leading etiologic agent of viral gastroenteritis in infants and young children worldwide1
Rotaviruses are a major cause of acute gastroenteritis in children under 5 years of age worldwide and rotavirus infections are responsible of approximately 128.000 deaths per year13
In the present study the reactivity of 88 serum samples from adults and children was analysed against seven different rotavirus VP8* proteins from different P genotypes, some from common rotavirus genotypes, P[4], P[6], P[8] and P[9] but others from uncommon such as P[11], P[14] and P[25]15
Summary
Rotaviruses are the leading etiologic agent of viral gastroenteritis in infants and young children worldwide. Several studies suggested that non-secretor status reduces the susceptibility to RV infections mostly related to the P[8], P[4] and P[6] rotavirus genotypes. Sero-epidemiological studies are of importance to elucidate the different viral agents and genotypes that are circulating in a particular area, for this reason the main aim of this work was to determine the serum antibody prevalence and titer to a panel of VP8* proteins from different rotavirus genotypes (P[4], P[6], P[8], P[9], P[11], P[14] and P[25]) in children and adults. The second aim was to determine if the secretor status (FUT2G428A polymorphism) was related to differential antibody response as a measure of susceptibility to the different rotavirus genotypes utilised here
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