Serjania marginata Casar. Hydroalcoholic Extract Reduced Cytokine and Inflammatory Parameters in Experimental Models of Inflammation and Infection in Mice

Abstract

Pharmacognosy Research,2022,14,2,127-134.DOI:10.5530/pres.14.2.18Published:April 2022Type:Original ArticleAuthors:Maicon Matos Leitão, Joyce Alencar Santos Radai, Luis Fernando Benitez Macorini, Thiago Leite Fraga, Silvia Cristina Heredia-Viira, Claudia Andrea Lima Cardoso, Arielle Cristina Arena, and Candida Aparecida Leite Kassuya Author(s) affiliations:Maicon Matos Leitão1,2,*, Joyce Alencar Santos Radai1, Luis Fernando Benitez Macorini1, Thiago Leite Fraga1, Silvia Cristina Heredia-Vieira3, Claudia Andrea Lima Cardoso4, Arielle Cristina Arena5, Candida Aparecida Leite Kassuya1 1School of Health Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul State, BRAZIL. 2School of Health Sciences, Unigran Capital University Center, Campo Grande, Mato Grosso do Sul State, BRAZIL. 3Center of Health Sciences, Anhanguera- Uniderp University, Campo Grande, Mato Grosso do Sul State, BRAZIL. 4Center of Studies on Natural Resources, Mato Grosso do Sul State University, Dourados, Mato Grosso do Sul State, BRAZIL. 5Department of Morphology, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL. Abstract:Objectives: This study investigated the antimycobacterial, anti-inflammatory and antihyperalgesic effects of hydroalcoholic extract from leaves of S. marginata (EESM) in in vitro and in vivo models. Methods and Results: EESM (0.98–1000 μg/ml) was evaluated in in vitro against Mycobacterium tuberculosis, M. bovis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis. The EESM oral administration (p.o.) (30, 100 and 300 mg/kg) and dexamethasone subcutaneous injection (s.c.) (1 mg/kg) were tested against the carrageenan-induced inflammatory paw edema and pleurisy in Swiss mice. The EESM (30 and 100 mg/kg, p.o.) and dexamethasone (1 mg/kg, s.c.) were tested against the CFA-induced paw inflammation and M. bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice. The minimum inhibitory concentration (MIC) of EESM in the presence of M. tuberculosis was 62.4 μg/ml. The values of MIC of EESM in the presence S. epidermidis, K. pneumoniae were 1000 μg/mL while EESM did not interfere against P. aeruginosa growth. EESM significantly inhibited paw edema/mechanical hyperalgesia in carrageenan induced paw inflammation and leukocytes migration/proteins exudation in carrageenan-induced pleurisy model. In the BCGinduced pleurisy model, the daily treatment for 7 days, with EESM inhibited the levels of IL-1β in blood and in pleural exudate. The EESM did not alter the mycobacterial growth in the cell culture from pleural lavage, spleen and liver samples collected from BCG-treated animals. The EESM significantly inhibited the persistent edema and mechanical hyperalgesia induced by CFA. Conclusion: This study confirms the EESM anti-inflammatory property and showed that EESM has high potency in inducing inhibition of mycobaterial growth and low potency or no effects in relation to other microorganisms. Key words: Mycobacterium tuberculosis, Bacillus Calmette-Guerin (BCG), Cipó-timbó, Inflammation, Tuberculosis. View:PDF (455.97 KB)