Serine214 of Ras2p plays a role in the feedback regulation of the Ras-cAMP pathway in the yeast Saccharomyces cerevisiae

Abstract

In the yeast Saccharomyces cerevisiae, Ras proteins are essential for the Ras-cAMP signaling pathway. A serine to alanine substitution at position 214 in the yeast Ras2p resulted in enhanced sensitivity to heat shock, reduced levels of storage glycogen and enhanced both basal cAMP level and glucose-induced cAMP signal. Further work showed that Ras2Ala214p had a higher GTP-binding capability than wild type Ras2p. These results suggested that serine 214 of Ras2p plays a role in the feedback regulation of the Ras-cAMP pathway.