Serial analysis of matrix metalloproteinase-2 in dialysate of rat sclerosing peritonitis models

Abstract

Background. Sclerosing peritonitis (SP) is a serious complication of continuous ambulatory peritoneal dialysis (CAPD). In order to carry out CAPD safely, it is important to analyze the development of SP. Methods. We prepared animal models of SP by the intraperitoneal administration of chlorhexidine gluconate (CHX) or talc, using male Sprague-Dawley rats. The peritoneal equilibration test and histological examinations were performed in the model rats, and dialysate drained from them was analyzed by gelatin zymography. Results. In the two types of SP animal models, matrix metalloproteinase-2 (MMP-2) level in dialysate correlated with the changes of inflammation, thickness of peritoneum, D/D0 glucose level (glucose level of drained dialysate obtained 90 min after the injection of 2.5% glucose containing peritoneal dialysis fluid divided by that obtained immediately after the injection), and net ultrafiltration. Conclusions. From these results, MMP-2 level in drained dialysate was considered to change with the development and progression of SP in rat models. Thus, MMP-2 has potential as a diagnostic marker for SP.