Serglycin promotes proliferation, migration, and invasion via the JAK/STAT signaling pathway in osteosarcoma.

Bin Lv,Renfeng Liu,Xiaofeng Tang,Guangyu Gao,Yiping Liang,Zhiping Zhang,Yuhong Guo,Zhengzai Dai,Cheng Ju,Xiao-Bin Lv,Min Tang

doi:10.18632/aging.203392

Abstract

Background: Osteosarcoma (OS) is a common disease in the world, and its pathogenesis is still unclear. This study aims to identify the key genes that promote the proliferation, invasion, and metastasis of osteosarcoma cells.Method: GSE124768 and GSE126209 were downloaded from the Gene Expression Omnibus (GEO) database. The gene ontology and enrichment pathway were analyzed by FunRich software. qPCR and Western blot were used to detect the gene expression. After gene knockdown, Transwell and wound healing assays were conducted on osteosarcoma cells to detect whether the genes were defined before enhancing the invasion of osteosarcoma.Results: Totally, 341 mRNAs were found to be regulated differentially in osteosarcoma cells compared to osteoblasts. In addition, the expression level of Serglycin (SRGN) in osteosarcoma cells was higher than that in human osteoblasts. The invasion and proliferation ability of osteosarcoma cells with upregulated Serglycin was significantly increased, and on the contrary, decreased after Serglycin knockdown. Moreover, we preliminarily found that Serglycin may associate with the JAK/STAT signaling pathway.Conclusions: By using microarray and bioinformatics analyses, differently expressed mRNAs were identified and a complete gene network was constructed. To our knowledge, we describe for the first time Serglycin as a potential biomarker.

Highlights

Osteosarcoma (OS) represents the most common malignant bone tumors with high metastatic potential and poor prognosis in adolescents under the age of 20 [1]
KEGG pathway analysis showed that these potential target genes were mainly enriched in 6 pathways including the PPAR signaling pathway, Peroxisome, Hippo signaling pathway, Tight junction, HIF-1 signaling pathway, and Pathogenic Escherichia coli infection (Figure 2B)
We identified 352 gene expression patterns related to cancer, indicating that these 352 genes play an important role in promoting the development of osteosarcoma

Summary

Introduction

Osteosarcoma (OS) represents the most common malignant bone tumors with high metastatic potential and poor prognosis in adolescents under the age of 20 [1]. It usually occurs in rapidly developing bones, such as the humerus, distal femur, or proximal tibia. It is a highly aggressive malignant tumor, often www.aging-us.com metastasizing to the lungs and other organs [2]. The invasion and proliferation ability of osteosarcoma cells with upregulated Serglycin was significantly increased, and on the contrary, decreased after Serglycin knockdown. We describe for the first time Serglycin as a potential biomarker

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Conclusion