Sequestration of Aeromonas hydrophila for Serine Protease Gene Detection From DiarrhoeaI Samples Through PCR

Abstract

Statement of the problem: Diarrhoea disease is an significant cause of morbidity and mortality in developing countries, predominantly in newborns and elders. Diarrhoea is caused by viral, bacterial, and parasitic taints, as well as food bigotries, response to medicines, and other physiological and immunological disorders (Tianyan song et al., 2004).In the last decades, Aeromonas have been progressively acknowledged as relevant etiological agents in gastrointestinal taints, as well as extraintestinal taints, septicemia, Urinary tract taints. Aim: To identify the serine protease gene from Aeromonas hydrophila from diarrheal sample by using PCR method Methodology: Aeromonas hydrophila is impervious to chlorine, freezing or cold temperatures. Aeromonas hydrophila can be ingested through fodder products that have already been crawling with the bacterium, such as sea food, meats and even certain vegetables such as sprouts. The virulence of Aeromonas is multi factorial, including adhesions, S-layer, lipopolysaccharides, siderophores and an assortment of exoenzymes and exotoxins, i.e., aerolysin/haemolysin, lipases and proteases.. Strains producing S-layer are more pathogenic for fish, but the role of S-layer in human taints is not clear.Serine protease (22 KDa) was stable at 56 0 C for 10 minutes, possessed cytotoxic activity and had an LD 50 of 150ng/g fish.) (Rodriguez et al., 2004). Polymerase chain reaction is a sensitive and specific method for identification of virulence gene. Inference: Hence for the direct detection of pathogenic Aeromonas species isolates, virulence determinants are used as a genetic marker. Thus PCR method is used to spot the contagious gene encoding serine protease using precise primers.