SEQUENTIAL SYNTHESES OF VARIOUS MOLECULAR SPECIES OF RIBONUCLEIC ACID IN THE EARLY PHASE OF CONIDIA GERMINATION IN ASPERGILLUS ORYZAE

Abstract

Ribonucleic acid synthesis in early stages of germination of Aspergillus oryzae conidia was studied using orthophosphate 32P or 14CO2 as precursor. RNA was fractionated by the methylated albumin-coated kieselguhr column chromatography or by the sucrose density gradient centrifugation analysis.RNA synthesis occurred at any time of germination so far observed and rRNA or its precursor was synthesized at the initial step of germination. sRNA synthesis occurred a little latter than the onset of germination and mRNA synthesis became apparent after 60min of incubation. Even in non-germinating medium (phosphate buffer), conidia could synthesize rRNA. It was suggested that the synthesis of rRNA or its precursor may be one of the starting reactions being driven at the very initial step of germination.