Sequential Substrate Removal in a Dilute System by Heterogeneous Microbial Populations

Abstract

The sequential metabolism of substrates by heterogeneous bacterial populations has been previously reported from this laboratory in studies with high substrate concentrations. This phenomenon has now been shown to occur at very dilute substrate concentrations, i.e., 5 mg/liter of glucose plus 5 mg/liter of sorbitol, in studies conducted under the conditions of the standard biochemical oxygen demand (BOD) test. Sequential metabolism of these substrates resulted in a diphasic curve of accumulated oxygen uptake wherein the two phases were separated by a discernible plateau. These findings illustrate one possible explanation for the generation of discontinuity in the kinetic course of carbonaceous BOD exertion.