Abstract
Sequential measurements were made of LH concentrations in systemic blood and of progesterone and 20α-OH-pregn-4-en-3- one secretion rates into ovarian venous blood of female rats on the afternoon of proestrus. On the days of estrus, diestrus I and diestrus II, evaluation was limited to a single time period between 3:30 and 5:00 PM. LH was quantitated by double antibody radioimmunoassay. Progesterone and 20α-OH-pregn-4-en-3-one were measured by gasliquid chromatography following purification through thin-layer chromatography. Elevations in LH level were observed as early as 2:00 PM and as late as 8:00 PM on the day of proestrus (uterine ballooning). On the days of estrus, diestrus I and diestrus II, LH remained at basal levels. No change in progesterone secretion occurred between diestrus II and 4 : 3 0 PM of proestrus. Secretion rates (μg/hr/ovary) of progesterone rose sharply after 4:30 PM and exhibited peak levels (40-fold change) at 8:00 PM. 20a-OHpregn- 4-en-3-one secretion rates (μg/hr/ovary) were hi...
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