Abstract
The present work attempts to utilize the potato powder as a low cost carbon source and statistically evaluate the medium composition for red pigment production by Monascus purpureus. Plackett–Burman (PB) design was successfully used to screen the significant medium variables and potato powder was found more significant than other components. Central composite design (CCD) of response surface methodology (RSM) was used to optimize the four significant medium components (Potato powder, K2HPO4, ZnSO4·7H2O and MSG) selected from PB experiment. ANOVA results showed that model was significant and R2 value of 0.833 demonstrated that experimental results were fitted well with predicted values. Maximum pigment absorbance of 7.18ODU/ml was predicted at optimal level of potato powder 2.50% (w/v), K2HPO4 0.480% (w/v), ZnSO4·7H2O 0.0013% (w/v) and MSG 0.60% (w/v). Model accuracy was found to be 96.65% from the data obtained from validation experiment.
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