Sequential in vitro reactivity of lymphocytes from patients with cervical squamous malignancy in a cytotoxicity assay

Abstract

The in vitro reactivity of peripheral blood lymphocytes from patients with cervical squamous malignancy was prospectively followed over a relatively long period of time. In 12 of 14 patients with preinvasive cervical lesions, reactivity was present at the time of initial diagnosis. Three months after treatment, reactivity was still present in only one of 12 (8%). Six months after the treatment, no significant reactivity could be detected in any of them. In the group with invasive squamous cell carcinoma, four patients developed recurrence in the course of follow-up. Reactivity was present in all at the time of initial diagnosis. In three of them, cytotoxic reactivity was retained up to the time of death in spite of clinical deterioration. One lost the reactivity after the third course of chemotherapy. Fourteen patients with invasive squamous cell carcinoma were followed up to 24 months without any evidence of recurrence. In all of this group, the cell-mediated cytotoxicity was nonreactive at 9 months. We conclude that: (1) patients with squamous cell carcinoma of the cervix demonstrated cell-mediated immune responses which disappeared 3 to 9 months after effective treatment (apparent cure) and (2) with persistent disease, in spite of marked clinical deterioration and inanition, cell-mediated cytotoxicity was demonstrable until the time of death.