Abstract
The detection of more than 1 antigen within a single tissue specimen is sometimes required in immunohistochemical staining. Several methods have been described as how to achieve this. In this experiment, a sequential doublestaining was performed, using enhanced polymer technology. The use of the Envision polymer technology combines a less complicated protocol with a high sensitivity and low background signal. Staining was performed on neutral buffered, formalin fixed, paraffin embedded human tissues of spleen, lymph node, follicle center cell lymphoma, and plasmacell infiltrate of a jaw cyst using antibodies against CD20, CD43, kappa light chains, and lambda light chains. Two vizualization systems were used, based on horseradish peroxidase-DAB and alkaline phosphatase-Fast Red. Staining patterns, color distribution, and practical approach are described in this article. (The J Histotechnol 24:107, 2001)Submitted: March 5, 2001; Accepted with revisions: March 8, 2001
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