Abstract
This study evaluated simultaneously the raw vinasse degradation, an effluent from the sugar-alcohol industry, the laccase production by Pleurotus sajor-caju and its purification using aqueous two-phase systems (ATPS). To improve laccase production, different concentrations of inducers (ethanol and CuSO4) were added. The higher laccase production promoted an increase of 4-fold using 0.4 mM of CuSO4 as inducer, with maximum enzymatic activity of 539.3 U/L on the 3rd day of fermentation. The final treated vinasse had a decolorization of 92% and turbidity removal of 99% using CuSO4. Moreover, the produced laccase was then purified by ATPS in a single purification step, reaching 2.9-fold and recovered ≈ 99,9 %, in the top phase (PEG-rich phase) using 12 wt% of PEG 1500 + 20 wt% of citrate buffer + enzyme broth + water, at 25 °C. Thus, an integrated process of vinasse degradation, laccase production and purification with potential industrial application was proposed.
Highlights
The sugarcane industry is an important economic segment and well developed in Brazil, making sugarcane ethanol the most successful alternative fuel program ever developed in the world [1]
Raw vinasse is mainly composed of water, inorganic minerals, suspended solids and organic pollutants such as phenolic and melanoidin compounds
This section describes the materials and main procedures used in the production, induction and purification of P. sajor-caju laccase
Summary
The sugarcane industry is an important economic segment and well developed in Brazil, making sugarcane ethanol the most successful alternative fuel program ever developed in the world [1]. Raw vinasse is mainly composed of water, inorganic minerals, suspended solids and organic pollutants such as phenolic and melanoidin compounds. It has a brown color, a corrosive low pH (3.5 – 5.0), a high chemical oxygen demand (70 – 150 g/L) and biochemical oxygen demand of 35 – 50 g/L. These characteristics make vinasse a complex residue to decompose [4] and its intensified use in agriculture cause nutrient saturation and pH increase, but it still is used as a fertilizer for sugarcane crop production
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