Sequential assignment of the 1H and 31P resonances of the double stranded deoxynucleotide d(ATGCAT) 2 by 2D-NMR correlation spectroscopy

Abstract

31P- 1H and 1H- 1H chemical shift correlation spectroscopy are jointly used for providing a complete assignment of sugar proton (except H 5′ and H 5″) and phosphorus resonances in the double stranded oligonucleotide d(ATGCAT) 2. In contrast to previous methods the specific assignment of overcrowded H 5′ H 5″ proton resonances is not required. Using the H 3′-P coupling and also the long range H 4′-P coupling, this quite general method can be easily implemented on intermediate field spectrometer. The present results pave the way to the 1H and 31P resonance assignment of longer double-stranded oligonucleotides.