Abstract
The present study was mainly focused on effective utilization of holocellulose content of cotton stalk for bioethanol production. Steam exploded cotton stalk (180 °C for 5 min; severity factor- 3.05) was subjected to sequential acid and enzymatic saccharification. Acid saccharification (1.5% H2SO4 at 110 °C for 60 min) of steam exploded cotton stalk released maximum of 37.24 ± 1.20 g/L reducing sugars with 69.16 ± 1.18% holocellulose hydrolysis. Subsequent enzymatic saccharification of steam exploded acid treated residue yielded maximum 8.50 ± 0.57 g/L sugar concentration with 84.20 ± 0.34% combined saccharification efficiency using 20 FPU/g cellulase loading (Accellerase- 1500) after 72 h incubation period. Finally, batch fermentation of mixed (acid-enzymatic) hydrolysate containing 45.74 ± 1.68 g/L sugars with Scheffersomyces stipitis NCIM 3498 produced 19.08 ± 0.56 g/L ethanol with 0.47 g/g ethanol yield after 48 h time period.
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