Abstract
The International Crocodilian Genomes Working Group (ICGWG) will sequence and assemble the American alligator (Alligator mississippiensis), saltwater crocodile (Crocodylus porosus) and Indian gharial (Gavialis gangeticus) genomes. The status of these projects and our planned analyses are described.
Highlights
Background on crocodilians and project justification The order Crocodylia, which typically refers to the clade that includes the extant crocodilians [20], is an ecologically successful group of reptiles that originated in the mid- to upper-Cretaceous period [21,22]
To provide the genomic resources necessary to expand our under standing of these fascinating organisms, the International Crocodilian Genomes Working Group (ICGWG) is obtaining and assembling genome sequences for the American alligator, saltwater crocodile, and gharial, one representative from each of the extant crocodilian families
To sequence the saltwater crocodile genome, we are combining high coverage Illumina short insert sequen cing with low coverage 454 libraries in a hybrid approach, similar to that used for the turkey genome [10]
Summary
Background on crocodilians and project justificationThe order Crocodylia, which typically refers to the clade that includes the extant crocodilians [20], is an ecologically successful group of reptiles that originated in the mid- to upper-Cretaceous period (approximately 100 Mya) [21,22]. To provide the genomic resources necessary to expand our under standing of these fascinating organisms, the ICGWG is obtaining and assembling genome sequences for the American alligator, saltwater crocodile, and gharial, one representative from each of the extant crocodilian families. Our efforts to identify novel repeats in preliminary saltwater crocodile and American alligator genome assemblies show that the most abundant repeats in the current assemblies are less than 1kbp (Figure 2).
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