Sequencing of Transcriptome-Wide Poly(A) Tails by PAIso-seq.

Yusheng Liu,Jiaqiang Wang,Falong Lu

doi:10.1007/978-1-0716-3481-3_13

Sequencing of Transcriptome-Wide Poly(A) Tails by PAIso-seq.

Yusheng Liu, Jiaqiang Wang + Show 1 more

https://doi.org/10.1007/978-1-0716-3481-3_13

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Journal: Methods in molecular biology (Clifton, N.J.)	Publication Date: Oct 13, 2023
Citations: 2

Affiliation: Northeast Forestry University, Northeast Agricultural University, University of Chinese Academy of Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

#Eukaryotic mRNA #Full-length cDNA + Show 8 more

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Abstract

Poly(A) tails are added to most eukaryotic mRNA and have essential regulatory functions. However, due to its homopolymeric nature, the sequence information in poly(A) tails is challenging to obtain in transcriptome measurement studies. In this chapter, we describe the detailed procedures of poly(A) inclusive full-length RNA isoform-sequencing (PAIso-seq), a method that can measure transcriptome-wide poly(A) tails from as low as nanogram level of total RNA based on the PacBio HiFi sequencing platform. The accurate length and base composition of poly(A) tails can be obtained along with the full-length cDNA.

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