SEQUENCING OF THE GENOME BACILLUS PUMILUS ONU 554 ISOLATED FROM DEEP WATER SEDIMENTS OF BLACK SEA

Abstract

The aim of the work was to analyse the structure of the genome of bacteria of the strain Bacillus sp. ONU 554 for its identification and revealing of the genes which are responsible for production of biologically active compounds. Materials and methods. The object of the study were structure and characteristics of antagonistically active bacteria of the strain Bacillus pumilus ONU 554. Genomic DNA was sequenced using the sequencer NiSeq 1500 (Illumina). The genome was assembled using the Newbler assembler version 2.8, the species of the strain was determined using a TYGS server, and the ANI was calculated using Ezbiocloud. Genome annotation was performed using PATRIC and NCBI PGAP servers, and clusters of antibiotic and bacteriocin biosynthesis were searched using antiSMASH and Bagel4, respectively. Search for determinants of pathogenicity was performed using IslandViewer, antibiotic resistance - ResFinder-3.2, search for prophage elements using PHASTER. Results. According to whole genome comparison and 16S rRNA of Bacillus sp. ONU 554 was identified as Bacillus pumilus ONU 554. Its genome is 3,642,544 bp in size, which is 90 kb smaller than the genome of a type strain due to a number of deletions. A plasmid, four prophage elements, one of which is on the plasmid, and 14 biosynthetic clusters, three of which belong to bacteriocins, were identified. Conclusions. Thus, we can conclude that whole-genome sequencing data are a more reliable tool for species identification than analysis of the total fatty acid profile, and the strain whose genome was analyzed can become an object for further molecular biotechnological work on the exploration and heterological expression of the identified clusters.