Sequencing of complete mitochondrial genome of brown algal Saccharina sp. ye-C6

Le Xu,Shuai Wang,Xiao Fan,Dong Xu,Xiaowen Zhang,Yu Miao,Naihao Ye

doi:10.3109/19401736.2015.1079880

Abstract

The complete mitochondrial sequence (37 657 bp) of the Saccharina sp. ye-C6 was obtained from the assembled and annotated genome data sequenced by Illumina sequencing technology (KT271760). The circular genome contains 38 protein-coding genes (PCG), three ribosomal RNA (rRNA), and 25 transfer RNA (tRNA) genes. Base composition of genome is A (28.41%), T (36.29%), C (14.72%), and G (20.58%). Through a phylogenetic analysis based on the mitochondrial genomes of brown algae, we found that Saccharina sp. ye-C6 and Saccharina japonica are the most closely related species and strongly supports their close phylogenetic affinity.

Highlights

The genome contains 38 protein-coding genes (PCG), three ribosomal RNA, 25 transfer RNA genes and the phylogenetic analysis based on the mitochondrial genomes of brown algae indicated that Saccharina sp. ye-C12 and Saccharina japonica are the most closely related species, which strongly supports their close phylogenetic affinity
The genome of S. japonica has been sequenced (Ye et al. 2015), which would powerfully push the genetic improvement of the species
Strains of Saccharina collected from different area all over the world were resequenced, which make us have opportunity to uncover some mitochondrial genomes of these strains to develop more genetic tools due to the enough illumina sequencing data

Summary

Introduction

The complete sequence (37 654 bp) of the mitochondrial DNA (mtDNA) of the Saccharina sp. Ye-C12 was determined using Illumina sequencing data. The genome contains 38 protein-coding genes (PCG), three ribosomal RNA (rRNA), 25 transfer RNA (tRNA) genes and the phylogenetic analysis based on the mitochondrial genomes of brown algae indicated that Saccharina sp. Ye-C12 and Saccharina japonica are the most closely related species, which strongly supports their close phylogenetic affinity.

Results

Conclusion