Abstract
A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTSI gene was obtained. Analysis of the ADAMTSI gene using the porcine radiation hybrid panel indicated that pig ADAMTSI is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTSI as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).
Highlights
A disintegrin-like and metalloprotease with thrombospondin type 1 motif (ADAMTS) is a novel family of extracellular proteases found in both mammals and invertebrates (Tang, 2001)
The purpose of our research was to characterise the pig ADAMTS1 gene and determine whether single nucleotide polymorphisms (SNP) of exon 7 in porcine ADAMTS1 gene are associated with litter size
The hydropathy plot and the TMAP prediction derived from the World-Wide Web service showed that porcine ADAMTS1 protein included no transmembrane region and
Summary
A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS) is a novel family of extracellular proteases found in both mammals and invertebrates (Tang, 2001). ADAMTS1 was first cloned in the cachexigenic colon 26 adenocarcinoma subline in vivo (Kuno et al, 1996). Later research showed that ADAMTS1 plays an important role in follicles via progesterone receptor (PR)-dependent pathways (Robker et al, 2000). Female fertility is impaired in ADAMTS1 knockout mice, and this is accompanied by obvious abnormalities. Variations in ADAMTS1 gene could be used as genetic markers to select animals for breeding. There is still no report on how a genetic variant of this gene affects litter size in pigs. The purpose of our research was to characterise the pig ADAMTS1 gene and determine whether single nucleotide polymorphisms (SNP) of exon 7 in porcine ADAMTS1 gene are associated with litter size
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