Abstract
Mastitis is considered the most predominant production infectious disease in dairy industry all over the world including Egypt, The difficulty of mastitis occurs due to its chronic nature of the disease, extremely economic losses, extensively spread all over the year and intense decrease in the milk production. Staphylococcus aureus is one of the most common bacterial agents causing mastitis and having public health importance. A total of 248 milk samples were collected from different dairy farms, 77 samples from mastitic milk, 171 from apparently normal milk. 24 samples were positive for S. aureus by applying coagulase test. This study is concerned with the detection of thermonuclease (NUC gene) of S. aureus through PCR reaction, sequencing and phylogenetic analysis. Sequence alignments and phylogenetic analysis revealed high level of similarities between the different alleles isolated from different samples. Genotyping and sequencing analysis of NUC gene is a good tool to understand the relationships and differences between different isolates of S. aureus causing mastitis in dairy farms.
Highlights
Mastitis is considered the most predominant infectious disease causing economic losses in dairy industry all over the world. (Seegers et al, 2003 and Petrovski et al, 2006)
Coagulase test: All staphylococci isolates were tested by slide coagulase test and tube coagulase test to differentiate between coagulase positive and coagulase negative S. aureus
polymerase chain reaction (PCR) product: PCR reaction revealed 21 positive amplicons of NUC gene and their length 267 bp in the presence of negative and positive controls which is characteristic for S. aureus
Summary
Mastitis is considered the most predominant infectious disease causing economic losses in dairy industry all over the world. (Seegers et al, 2003 and Petrovski et al, 2006). Mastitis is considered the most predominant infectious disease causing economic losses in dairy industry all over the world. The effective consequences of mastitis occur due to chronic nature of the disease, high economic losses, widely spread all over the year and dramatic decrease the milk productivity. Bacterial species are the most common pathogenic agents causing mastitis in bovine. The adherence of the bacterium to the epithelial cells of the mammary gland make it difficult to be washed away. It secretes different factors through which decreasing the phagocytic activity like protein A, capsuleand pseudo capsule (Sutra and Poutrel, 1994)
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