Abstract
ABSTRACTThe solute carrier family 11 member A1 (SLC11A1), also known as natural resistance-associated macrophage protein 1 (NRAMP1), is a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. To study the genetic diversity of the SLC11A1 gene in Doom pigs of Assam especially on its evolution and differentiation within and among species, the partial sequence of the SLC11A1 gene was sequenced, characterized and compared with other published sequences of pigs and other species livestock. The gene sequence of Doom pigs showed the highest sequence identity with EF200584.1 (exotic pig) and the lowest similarity AY368475.1 (large white strain 008). One single nucleotide polymorphism (SNP) was identified in the heterozygous sequence at the 736 bp position (A→G). The sequence showed the highest sequence identity (81.74%) with that of O. aries and the lowest similarity (39.12%) with B. bubalis (Mehsana breed), respectively. Phylogenetic analysis revealed that the Doom pig is more closely related to EU135795.1 (Chinese local pig) and EF200584.1 (Pig, Iowa State University). The sequencing and phylogenetic analysis of the SLC11A1 gene of Doom pigs could be used for genetic selection with disease-resistance varieties and upgradation of indigenous germplasm of domestic livestock.
Highlights
Pig husbandry is of crucial importance in providing nutritional security and gainful employment to the weaker sections of society
The solute carrier family 11 member A1 (SLC11A1) gene, formerly known as natural resistance-associated macrophage protein 1 (NRAMP1) encodes an integral membrane protein regulating the activity of macrophages (Blackwell 1996; Vidal et al 1996; Forbes & Gros 2003)
All the nucleotides of SLC11A1 genes obtained in the present study showed a 50–99.2% similarity with the other pigs
Summary
Pig husbandry is of crucial importance in providing nutritional security and gainful employment to the weaker sections of society. The solute carrier family 11 member A1 (SLC11A1) gene, formerly known as natural resistance-associated macrophage protein 1 (NRAMP1) encodes an integral membrane protein regulating the activity of macrophages (Blackwell 1996; Vidal et al 1996; Forbes & Gros 2003). The gene codes for an iron/divalent cation transporter (Blackwell et al 2003) confer resistance to a number of antigenically different intracellular pathogens namely Salmonella typhimurium, Leishmania donovani and Mycobacterium bovis in mice (Vidal et al 1993; Xiaoling et al 2014). The report on phylogenetic analysis and sequencing of disease- resistance genes are very few in domestic animals. Keeping in view the above facts, the present study has been conducted to sequence and study the phylogenetic relationship of the SLC11A1 gene as a potent candidate gene for disease resistance in indigenous Doom pigs of Assam
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