Abstract
The complete mitogenome sequence of the masked shrew (Sorex caecutiens) was determined using long PCR. The genome was 17,096 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one origin of L strand replication, and one control region. The overall base composition of the heavy strand is A (32.9%), C (24.5%), T (29.3%), and G (13.3%). The base compositions present clearly the A–T skew, which is most obviously in the control region and protein-coding genes. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of the masked shrew. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. The five Sorex species formed a monophyletic group with the high bootstrap value (100%) in all examinations.
Highlights
The complete mitogenome sequence of the masked shrew (Sorex caecutiens) was determined using long PCR
A muscle sample was obtained from a female the masked shrew captured from Luobei region of Small Khingan Mountains in Heilongjiang Province, China (455000700 N, 1328407400 E)
Three domains were defined in the masked shrew mitochondrial genome control region (Zhang et al 2009): the extended termination-associated sequence (ETAS) domain, the central conserved domain (CD), and the conserved sequence block (CSB) domain
Summary
The complete mitogenome sequence of the masked shrew (Sorex caecutiens) was determined using long PCR. KEYWORDS Control region; mitogenome; phylogenetic trees; masked shrew The complete mitochondrial genome of the masked shrew (Sorex caecutiens) was sequenced for the first time on ABI 3730XL using a primer walking strategy and the long and accurate PCR, with five pairs of long PCR primers and with 14 pairs of sub-PCR primers.
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