Abstract
The soluble methane monooxygenas (sMMO) genes and 16S rDNA of Methylosinus trichosporium IMV 3011 were sequenced. Sequence comparison of the sMMO genes between strain IMV 3011 and Methylosinus trichosporium OB3b showed 99.0% to 82.7% identity, and the comparison of deduced amino acid sequences were 65.1–99.4% identity. The translated MMOX sequence comparison showed 99.4–81.8% identity to the corresponding sequence of four published MMOX sequences. Analysis of the active site showed highly conserved diiron centers in its α subunits. The multiple alignments of MMOX sequences exhibited a good conservative characteristic. Phylogenetic analysis of the 16S rDNA and MMOX sequences revealed that strain IMV 3011 is a true Methylosinus trichosporium, the closest species is Methylosinus trichosporium OB3b, and they have a similar activity site of sMMO.
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