Sequences of initiator and elongator methionine tRNAs in bean mitochondria

Abstract

Two bean mitochondria methionine transfer RNAs, purified by RPC-5 chromatography and two-dimensional gel electrophoresis, have been sequenced usingin vitro post-labeling techniques.One of these tRNAs(Met) has been identified by formylation using anE. coli enzyme as the mitochondrial tRNAF (Met). It displays strong structural homologies with prokaryotic and chloroplast tRNAF (Met) sequences (70.1-83.1%) and with putative initiator tRNAm (Met) genes described for wheat, maize andOenothera mitochondrial genomes (88.3-89.6%).The other tRNA(Met), which is the mitochondrial elongator tRNAF (Met), shows a high degree of sequence homology (93.3-96%& with chloroplast tRNAm (Met), but a weak homology (40.7%) with a sequenced maize mitochondrial putative elongator tRNAm (Met) gene.Bean mitochondrial tRNAF (Met) and tRNAm (Met) were hybridized to Southern blots of the mitochondrial genomes of wheat and maize, whose maps have been recently published (15, 22), in order to locate the position of their genes.