Abstract
Alanen, M., Wartiovaara, J. and Soderlund, H. 1987. Sequences conserved in the defective interfering RNAs of Semliki Forest Virus: An electron microscopic heteroduplex analvsis. - Hereditas 106: 19–29. Lund, Sweden. ISSN 00184661. The RNA structure of three species of defective interfering particles (DI) that have been generated by undiluted passaging of Semliki Forest virus was determined by heteroduplex analysis of Complementary DNA. Hybridization of the three cDNAs with two previously sequenced DI cDNA species leads to the elucidation of the sequences common to the five different DIs. There are altogether about 270 nucleotides in three separate sequence blocks (80, 90 and 100 nucleotides) that have been conserved through passaging. Signals for the process of replication and encapsidation must have been retained in these sequences. One of the sequences contains a 20 nucleotides long region with a palindromic sequence also found in the DI-RNAs of the closely related Sindbis virus.
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