Sequence-Controlled Stimuli-Responsive Single-Double Helix Conversion between 1:1 and 2:2 Chloride-Foldamer Complexes.

Abstract

The primary sequence in biopolymers carries the information to direct folded secondary structures, tomodulate their stabilities, and tocontrol the resultant functions. Our ability to encode such information into nonbiological oligomers and polymers, however, is still limited. Here, we describe a C2-symmetric aryl-triazole foldamer that assembles into a chloride-templated 2:2 double helix, and the discovery that its interconversion with the simpler 1:1 single helix can be driven by solvent quality, temperature, and concentration. We use single-site substitutions in the 13-residue sequence (two terminal sites and one central site) to reveal that the stability of the double helix is largely dictated by the differences in the anion binding power between single and double helices as well as the location of the modified residues. Specifically, placement of stabilizing CH···Cl- hydrogen-bonding interactions at the chain ends in the form of bisamide phenylene residues is found to highly favor the double helix. While the burial of π surfaces and the solvophobic effect also help to stabilize the double helix, their role was found to be less sensitive to the modifications considered. This understanding of how chemical information is programmed into the primary sequence provides a powerful tool for controlling structure and properties of abiological foldamers.