Abstract
Hepatitis E virus (HEV) is a major causative agent of acute hepatitis in developing countries. The Norway rat HEV genome consists of six open reading frames (ORFs), i.e., ORF1, ORF2, ORF3, ORF4, ORF5 and ORF6. The additional reading frame encoded protein ORF5 is attributed to life cycle of rat HEV. The ORFF5 protein’s function remains undetermined. Therefore, it is of interest to analyze the ORF5 protein for its physiochemical properties, primary structure, secondary structure, tertiary structure and functional characteristics using bioinformatics tools. Analysis of the ORF5 protein revealed it as highly unstable, hydrophilic with basic pI. The ORF5 protein consisted mostly of Arg, Pro, Ser, Leu and Gly. The 3D structural homology model of the ORF5 protein generated showed mixed α/β structural fold with predominance of coils. Structural analysis revealed the presence of clefts, pores and a tunnel. This data will help in the sequence, structure and functional annotation of ORF5.
Highlights
Hepatitis E virus (HEV) is the major aetiological agent of Hepa titis E, called enteric hepatitis infection [1 ].Worldwide, about 20 million HEV infections and 3.3 million symptomatic hepatitis E cases occur annually which results in 44,000 deaths [2]
The HEV genome is categorized into three open reading frames (ORFs): ORF1, ORF2 and ORF3
It was predicted through software 3D model was validated using Ramachandran plot analysis that the genome in these rat HEV sequences was organized into a (PROCHECK) for stereototal of six reading frames
Summary
Hepatitis E virus (HEV) is the major aetiological agent of Hepa titis E, called enteric hepatitis (enteric means related to the intestines) infection [1 ].Worldwide, about 20 million HEV infections and 3.3 million symptomatic hepatitis E cases occur annually which results in 44,000 deaths [2]. G3, HEV G4 and avian HEV [8] It was predicted through software 3D model was validated using Ramachandran plot analysis that the genome in these rat HEV sequences was organized into a (PROCHECK) (http://nihserver.mbi.ucla.edu/SAVES) for stereototal of six reading frames HEV genomic organization, the ORFs ORF1 and ORF3 do not protein was predicted using Signal P-4.1 Recent studies have elucidated the (http://www.cbs.dtu.dk/services/NetNGlyc/) server, provided by characteristics of some of the less understood ORF encoded Centre for Biological Sequence Analysis, Technical University of proteins using computational approaches to delineate their role in Denmark (CBS DTU). 4.0 analyzed the ORF5 protein for its physiochemical properties, (http://www.cbs.dtu.dk/services/NetOGlyc/) server, provided by primary structure, secondary structure, tertiary structure and Centre for Biological Sequence Analysis, Technical University of functional characteristics using bioinformatics tools. ANTHEPROT (National Center for Biotechnology Information) GenBank database. v.6.9.3 predicted phosphorylation and other modified sites in the ORF5 protein
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