Abstract
Non-glucosylated, non-methylated phage T2 DNA was methylated in vitro with partially purified wild-type ( dam +) or mutant ( dam h) T2 DNA adenine methylase. The radioactively labeled methyladenine-containing DNA was enzymatically degraded and the resulting oligonucleotides were separated according to chain length by DEAE-cellulose chromatography. Following “fingerprinting” by two-dimensional electrophoresis, we determined the sequence for various di-, tri- and tetranucleotides containing radioactive N 6-methyldeoxyadenosine. From this analysis we conclude that both T2 dam + and T2 dam h contain the sequence 5′…G-mA -Py…3′.
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