Sequence polymorphism and haplogroup data of the hypervariable regions on mtDNA in Semoq Beri population

Muhamad Aidil Zahidin,Wan Bayani Wan Omar,Wan Rohani Wan Taib,Jeffrine Rovie Ryan Japning,Mohd Tajuddin Abdullah

doi:10.1016/j.dib.2018.10.158

Muhamad Aidil Zahidin, Wan Bayani Wan Omar + Show 3 more

Open Access

https://doi.org/10.1016/j.dib.2018.10.158

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Abstract

Orang Asli is the aboriginal people in Peninsular Malaysia who have been recognized as indigenous to the country and still practicing traditional lifestyle. The molecular interest on the Orang Asli started when the earliest prehistoric migration occurred approximately 200 kya and entering Peninsular Malaysia 50 kya in stages. A total of three groups of Orang Asli present in Peninsular Malaysia, namely, Negrito also known as Semang, Senoi and Proto Malays. Through records, there is no research has been conducted on mtDNA variations in the Semoq Beri population, one of the tribes in Senoi group. In this report, variations of mtDNA were analysed in the population in Hulu Terengganu as an initial effort to establish the genetic characterisation and elucidating the history of Orang Asli expansion in Peninsular Malaysia. An array of mtDNA parameters was estimated and the observed polymorphisms with their respective haplogroups in comparison to rCRS were inferred respectively. The DNA sequences are registered in the NCBI with accession numbers KY853670-KY853753.

Highlights

The DNA sequences are registered in the NCBI with accession numbers KY853670-KY853753
ClustalW2 MUSCLE, MEGA 7 software [1], DnaSP 5.1 software [2] and Haplogroup software Raw and analysed Blood sample collection, DNA extraction, PCR amplification, DNA purification, sequencing and data interpretation Sequence analysed followed by haplogroup identification Kampung Sungai Berua, Hulu Terengganu, Terengganu, Malaysia The mtDNA sequences are registered in the NCBI with accession number KY853670-KY853753 [Table S1] Zahidin [3]
All sequence data were generated from DNA samples that were collected with informed and written consent, and approved by Universiti Sultan Zainal Abidin (UniSZA) Human Research Ethics Committee, Malaysia

Summary

C C C CCCCCCCCCCCCC

N - deletion base, ns - total number of sequences, n - total number of unbroken bases C series. Nucleotide position (%) Base pair No of polymorphic sites No of observed transitions No of observed transversions No of indels Nucleotide composition (%) C T A G. 72 to 351 (49%) 280 bp 26 17 9 5 27.66 27.39 28.75 16.20 jameslick.com) on HVSI sequences. The schematic diagrams that represent two major haplogroups, which are M and N, were drawn The schematic diagrams that represent two major haplogroups, which are M and N, were drawn ( Figs. 1 and 2)

Sample collection and genomic DNA extraction

Findings

Statistical sequence analyses