Sequence Length Variation and Transmembrane Analysis of Contagious Bovine Pleuropneumonia (CBPP) and Contagious Caprine Pleuropneumonia (CCPP) Proteins

Abstract

A total of forty contagious bovine pleuropneumonia CBPP and contagious caprine pleuropneumonia CCPP proteins comprising each of cattle and goats were retrieved from the GenBank www ncbi nlm nih gov The Genbank accession numbers of the sequences and sequence variations of the proteins were used to investigate the molecular identity of various CBPP and CCPP proteins The protein molecules of the CBPP and CCPP proteins had varied amino acid sequences This indicated that the genome that coded for the building of their protein molecule exhibited high level of polymorphism The CBPP and CCPP protein rsquo s amino acid sequences were subjected to transmembrane domain identification using TMbase The Transmembrane of CBPP revealed that the inside to outside and outside to inside are significant while that of CCPP inside to outside only sequence position from is significant and outside to inside only sequence position from is significant Phylogenetic trees analysis by Neighbor Joining NJ trees were constructed using CBPP and CCPP protein sequences The evolutionary distances were computed using the Poisson correction method The reliability of the trees was calculated by bootstrap confidence values with bootstrap iterations using MEGA software Similar CBPP and CCPP proteins tend to cluster together compared to proteins that are distantly related in both species This could be seen among others in the closeness of protein P Phosphoglycerate kinase bovine and KEY Phosphoglycerate kinase caprine The study concluded that new typing tool may help improve the surveillance and control of the disease as well as to trace new epidemics