Abstract
The neem tree has long been recognized for its unique properties, both against insects and in improving human health. Every part of the tree has been used as a traditional medicine for household remedy against various human ailments, from antiquity. Although the occurrence of various phytochemicals in neem has been studied, we have identified the presence of a novel tripeptide in the young leaves of neem using a simple and inexpensive paper chromatographic method, detected by Cu(II)-ninhydrin reagent. The peptide nature of the isolated compound is confirmed by spectral studies. The sequence of the peptide is determined using de novo sequencing by tandem MS after purification.
Highlights
Small alpha peptides are the most expensive substances, and most of them are not available commercially [1]
The plant extract was run on circular paper chromatography using isopropanol : water (4 : 1) solvent system and developed with Cu(II)-ninhydrin reagent
The production of yellow chromaphore indicated the presence of Cu(II)ninhydrin-positive compound
Summary
Small alpha peptides are the most expensive substances, and most of them are not available commercially [1]. Pharmacological studies have proved that many peptides, including those isolated from plants, have a potential antitumor effect [2]. All amino acids and their carboxyl group derivatives like esters and amides, including small peptides, produce a purple color with the classical ninhydrin reagent. Amino acids produce a pink color, and their carboxyl derivatives like esters and amides, including small peptides, produce a yellow color with Cu(II)ninhydrin reagent. The Cu(II)-ninhydrin method discussed here is a novel one because no other methods presently used can form two different coloured products with a single developing reagent We have used this method for the detection and purification of amino acid derivatives from. 3.284 isolation and sequence determination of a small alpha peptide from the young leaves of A. indica
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